Goat anti-Human Transferrin Antibody Biotinylated
Product Details
Specifications
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400
Request Formulation Change Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Request Formulation Change Serum containing 0.09% Sodium Azide
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Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human transferrin. This antibody may cross react with transferrin from other species. Goats were immunized with purified human transferrin of serum or plasma origin. Antiserum was solid phase adsorbed to ensure class specificity. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP).
Prior to conjugation, immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Molar enzyme/antibody protein ratio is 4:1.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human transferrin. This antibody may cross react with transferrin from other species. Goats were immunized with purified human transferrin of serum or plasma origin. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
By immunoelectrophoresis and ELISA this antibody reacts specifically with human transferrin. This antibody may cross react with transferrin from other species. Goats were immunized with purified human transferrin of serum or plasma origin. By immunoelectrophoresis, the antiserum reacts specifically with human transferrin. No antibody was detected against other human serum proteins.
This antibody may cross react with transferrin from other species.
Additional Product Information
Transferrin is a glycoprotein responsible for the transport of iron through the bloodstream. Produced in the liver, its levels can act as a general readout for liver function and health.
Alternate Names
beta-1 metal-binding globulin; epididymis secretory sperm binding protein Li 71p; HEL-S-71p; PRO1557; PRO2086; serotransferrin; siderophilin; TFQTL1; Transferrin
Applications
Not all listed applications have been specifically tested by our laboratory.
For use in precipitin gel reactions (e.g. immunoelectrophoresis (IEP) or double diffusion Ouchterlony (DD)).
Optimal working dilutions should be determined experimentally by the investigator.