Goat anti-RPA32 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-874A-T maps to a region between residue 220 and 270 of human Replication Protein A2 (32kD) using the numbering given in entry NP_002937.1 (GeneID 6118).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to RPA32 immobilized on solid support.
The epitope recognized by A303-874A maps to a region between residue 220 and 270 of human Replication Protein A2 (32kD) using the numbering given in entry NP_002937.1 (GeneID 6118).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Replication protein A (RPA) is a multisubunit complex that carries out DNA mismatch repair (MMR) in association with MSH2 (a subunit of human MutS heterodimers), MLH1 (a subunit of human MutL heterodimers), PCNA, and DNA polymerase-delta. RPA is composed of a heterotrimer that includes subunits of 70 kDa (RPA1), 32kDa (RPA2), and 14kDa (RPA3). RPA2, the 32kDa subunit, is phosphorylated by the cdc2 family of kinases when cells enter S-phase and in response to DNA damage by ATM, ATR, and DNA-PK.
Alternate Names
REPA2; replication factor A protein 2; replication protein A 32 kDa subunit; replication protein A 34 kDa subunit; RF-A protein 2; RP-A p32; RP-A p34; RPA32
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots are performed using Donkey anti-Goat IgG Heavy and Light Chain Antibody (Cat. No. A50-101P).