Rabbit anti-ACL Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-867A maps to a region between residue 1051 and 1101 of human ATP citrate lyase using the numbering given in entry NP_001087.2 (GeneID 47).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to ACL immobilized on solid support.
The epitope recognized by A303-867A-T maps to a region between residue 1051 and 1101 of human ATP citrate lyase using the numbering given in entry NP_001087.2 (GeneID 47).
Additional Product Information
ATP citrate lyase (ACL) is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. ACL is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ACL may be involved in the biosynthesis of acetylcholine [taken from NCBI Entrez Gene (Gene ID: 47)].
Alternate Names
ACL; ATP-citrate (pro-S-)-lyase; ATP-citrate synthase; ATPCL; citrate cleavage enzyme; CLATP
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).