Rabbit anti-ACO2/Aconitase Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-302A maps to a region between residue 350 to 400 of human Aconitate hydratase, mitochondrial using the numbering given in entry Q99798.2 (GeneID 50).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to ACO2/Aconitase immobilized on solid support.
The epitope recognized by A305-302A-T maps to a region between residue 350 to 400 of human Aconitate hydratase, mitochondrial using the numbering given in entry Q99798.2 (GeneID 50).
Additional Product Information
Aconitase belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification [[taken from NCBI Entrez Gene (Gene ID: 50)].
Alternate Names
Aconitase; aconitase 2, mitochondrial; aconitate hydratase, mitochondrial; ACONM; citrate hydro-lyase; epididymis secretory sperm binding protein Li 284; HEL-S-284; ICRD; mitochondrial aconitase; OCA8; OPA9
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.