Rabbit anti-ADA/Adenosine Deaminase Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-183A maps to a region between residue 100 to 150 of human Adenosine deaminase using the numbering given in entry P00813.3 (GeneID 100).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to ADA/Adenosine Deaminase immobilized on solid support.
The epitope recognized by A305-183A-T maps to a region between residue 100 to 150 of human Adenosine deaminase using the numbering given in entry P00813.3 (GeneID 100).
Additional Product Information
The gene ADA encodes the enzyme adenosine deaminase which catalyzes the hydrolysis of adenosine to inosine. Deficiency in this enzyme causes a form of severe combined immunodeficiency disease (SCID), in which there is dysfunction of both B and T lymphocytes with impaired cellular immunity and decreased production of immunoglobulins. Elevated levels of this enzyme have been associated with congenital hemolytic anemia [taken from NCBI Entrez Gene (Gene ID: 100)].
Alternate Names
adenosine aminohydrolase; adenosine deaminase
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.