Rabbit anti-AHI1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-301A maps to a region between residue 1146 and 1196 of human Abelson Helper Integration site 1 using the numbering given in entry NP_001128302.1 (GeneID 54806).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to AHI1 immobilized on solid support.
The epitope recognized by A303-301A-T maps to a region between residue 1146 and 1196 of human Abelson Helper Integration site 1 using the numbering given in entry NP_001128302.1 (GeneID 54806).
Additional Product Information
Abelson helper integration site 1 (AHI1) is also known as jouberin. AHI1 is apparently required for both cerebellar and cortical development in humans. Mutations in AH1 cause specific forms of Joubert syndrome-related disorders. Joubert syndrome (JS) is a recessively inherited developmental brain disorder with several identified causative chromosomal loci [taken from NCBI Entrez Gene (Gene ID: 55054)].
Alternate Names
abelson helper integration site 1 protein homolog; AHI-1; contatins SH3 and WD40 domains; dJ71N10.1; JBTS3; jouberin; ORF1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.