Rabbit anti-AIF Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-783A maps to a region between residue 563 and 613 of human Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 using the numbering given in entry NP_004199.1 (GeneID 9131).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to AIF immobilized on solid support.
The epitope recognized by A302-783A-T maps to a region between residue 563 and 613 of human Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 using the numbering given in entry NP_004199.1 (GeneID 9131).
Additional Product Information
Apoptosis-inducing factor (AIF) is a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, AIF induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9.
Alternate Names
AIF; apoptosis-inducing factor 1, mitochondrial; apoptosis-inducing factor, mitochondrion-associated, 1; CMT2D; CMTX4; COWCK; COXPD6; DFNX5; NADMR; NAMSD; PDCD8; programmed cell death 8 (apoptosis-inducing factor); Programmed cell death protein 8; striatal apoptosis-inducing factor; testicular secretory protein Li 4
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.