Rabbit anti-ANKRD13A Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-868A maps to a region between residue 500 and 550 of human Ankyrin repeat domain-containing protein 13A using the numbering given in entry Q8IZ07.3 (GeneID 88455).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to ANKRD13A immobilized on solid support.
The epitope recognized by A305-868A-T maps to a region between residue 500 and 550 of human Ankyrin repeat domain-containing protein 13A using the numbering given in entry Q8IZ07.3 (GeneID 88455).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
ANKRD13A is a ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. Does not bind 'Lys-48'-linked ubiquitin. Positively regulates the internalization of ligand-activated EGFR by binding to the Ub moiety of ubiquitinated EGFR at the cell membrane. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q8IZ07].
Alternate Names
ANKRD13; ankyrin repeat domain-containing protein 13A; NY-REN-25; NY-REN-25 antigen; Protein KE03
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.