Rabbit anti-ANKRD57 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-411A-T maps to a region between residue 75 and 125 of human ankyrin repeat domain 57 using the numbering given in entry NP_075392.2 (GeneID 65124). Antibody was affinity purified using an epitope specific to ANKRD57 immobilized on solid support.
The epitope recognized by A301-411A maps to a region between residue 75 and 125 of human ankyrin repeat domain 57 using the numbering given in entry NP_075392.2 (GeneID 65124).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
ANKRD57 is an ankyrin-repeat-containing protein whose function has not been characterized.
Alternate Names
ANKRD57; ankyrin repeat domain 57; ankyrin repeat domain-containing protein 57; ankyrin repeat domain-containing protein SOWAHC; C2orf26; protein sosondowah homolog C
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.