Rabbit anti-ARA70 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-272A maps to a region between residue 564 and 614 of human 70 kDa AR-activator (nuclear receptor coactivator 4) using the numbering given in entry NP_005428.1 (GeneID 8031).
Immunoglobulin concentration was determined using Beer��s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to ARA70 immobilized on solid support.
The epitope recognized by A302-272A-T maps to a region between residue 564 and 614 of human 70 kDa AR-activator (nuclear receptor coactivator 4) using the numbering given in entry NP_005428.1 (GeneID 8031).
Additional Product Information
ARA70 (androgen receptor-associated 70) was originally identified as part of chromosomal rearrangement involving the RET proto-oncogene. It was subsequently isolated in a two-hybrid screen as a ligand-dependent associated factor of the androgen receptor (AR). In addition to functioning as a co-activator of the AR, ARA70 has been found to function as a co-activator of the vitamin D receptor, estrogen receptor, PPARalpha, and PPARgamma.
Alternate Names
70 kDa androgen receptor coactivator; 70 kDa AR-activator; Androgen receptor coactivator 70 kDa protein; androgen receptor-associated protein of 70 kDa; ARA70; ELE1; NCoA-4; nuclear receptor coactivator 4; PTC3; ret fused; RET-activating gene ELE1; Ret-activating protein ELE1; RFG
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.