Rabbit anti-ARHGEF16 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-961A maps to a region between residue 175 and 225 of human Rho guanine exchange factor (GEF) 16 using the numbering given in entry NP_055263.2 (GeneID 27237).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to ARHGEF16 immobilized on solid support.
The epitope recognized by A301-961A-T maps to a region between residue 175 and 225 of human Rho guanine exchange factor (GEF) 16 using the numbering given in entry NP_055263.2 (GeneID 27237).
Additional Product Information
The gene for ARHGEF16 [Rho guanine exchange factor (GEF)16] was isolated as a candidate gene for human neuroblastoma. It is a member of the Rho/Rac GEF family of proteins which catalyze the exchange of GDP for GTP on the GTPases, Rho and Rac, to activate downstream signaling pathways.
Alternate Names
ephexin4; ephexin-4; GEF16; NBR; Rho guanine exchange factor (GEF) 16; Rho guanine nucleotide exchange factor (GEF) 16; rho guanine nucleotide exchange factor 16
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.