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Rabbit anti-ARHGEF5 IHC Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
ARHGEF5
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 600 and 650
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (50+ slides)

10 µl (5+ slides)

$131.00 $492.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Antigen Species
Human
Concentration
250 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to ARHGEF5 immobilized on solid support.

The epitope recognized by IHC-00652 maps to a region between residue 600 and 650 of human Rho guanine nucleotide exchange factor (GEF) 5 using the numbering given in entry NP_005426.2 (GeneID 7984).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to ARHGEF5 immobilized on solid support.

The epitope recognized by IHC-00652-T maps to a region between residue 600 and 650 of human Rho guanine nucleotide exchange factor (GEF) 5 using the numbering given in entry NP_005426.2 (GeneID 7984).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA

Additional Product Information

ARHGEF5, also known as TIM (transforming immortalized mammary), is an oncogene that belongs to the Dbl family of guanine nucleotide exchange factors (GEFs) for Rho GTPases. It is implicated to play a role in proliferative breast disease.

7984
ARHGEF5
Rho guanine nucleotide exchange factor 5
Q12774
Rho guanine nucleotide exchange factor 5

Alternate Names

ephexin3; ephexin-3; GEF5; guanine nucleotide regulatory protein TIM; oncogene TIM; P60; p60 TIM; Rho guanine nucleotide exchange factor (GEF) 5; rho guanine nucleotide exchange factor 5; TIM; TIM1; transforming immortalized mammary oncogene

Applications

Epitope exposure is recommended.

Epitope exposure with citrate buffer will enhance staining.

Likely to work with frozen sections.

In some cases, the antibody may be diluted further than indicated.