Rabbit anti-ARMS Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-002A maps to a region between residue 1400 and 1450 of human Ankyrin Repeat-Rich Membrane Spanning using the numbering given in entry NP_065789.1 (GeneID 57498).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to ARMS immobilized on solid support.
The epitope recognized by A303-002A-T maps to a region between residue 1400 and 1450 of human Ankyrin Repeat-Rich Membrane Spanning using the numbering given in entry NP_065789.1 (GeneID 57498).
Additional Product Information
Ankyrin repeat-rich membrane-spanning protein (ARMS) is also known as Kinase D-interacting substrate of 220 kDa (KIDINS220). ARMS Promotes prolonged MAP-kinase signaling by neurotrophins through activation of a Rap1-dependent mechanism. Arms may play an important role in regulating postsynaptic signal transduction through the syntrophin-mediated localization of receptor tyrosine kinases such as EPHA4. ARMS may play a role in neurotrophin- and ephrin-mediated neuronal outgrowth and in axon guidance during neural development and in neuronal regeneration. ARMS has also been shown to modulate stress-induced apoptosis of melanoma cells via regulation of the MEK/ERK signaling pathway [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q9ULH0].
Alternate Names
ankyrin repeat-rich membrane-spanning protein; ARMS; kinase D-interacting substrate 220kDa; kinase D-interacting substrate of 220 kDa; SINO
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.