Rabbit anti-AS160 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-727A maps to a region between residue 1 and 50 of human Akt Substrate of 160 kDa using the numbering given in entry NP_055647.2 (GeneID 9882).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to AS160 immobilized on solid support.
The epitope recognized by A303-727A-T maps to a region between residue 1 and 50 of human Akt Substrate of 160 kDa using the numbering given in entry NP_055647.2 (GeneID 9882).
Additional Product Information
The Akt substrate of 160 kDa (AS160) is a substrate of Akt kinase activity and is a GTPase activating protein (GAP) for Rab GTPases. AS160 is encoded by the TBC1D4 (TBC1 domain family, member 4) gene.
Alternate Names
akt substrate of 160 kDa; AS160; NIDDM5; TBC (Tre-2, BUB2, CDC16) domain-containing protein; TBC1 domain family member 4
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.