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Rabbit anti-ASH2 IHC Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
ASH2
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 578 and C-terminus
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (50+ slides)

10 µl (5+ slides)

$131.00 $492.00
Qty:

Product Details

Specifications

Verified Reactivity
Mouse, Human
Presumed Reactivity
Guinea Pig, Horse, Zebra Finch, Turkey, Dog, Chicken, Bovine, Monkey, Rat
Antigen Species
Human
Concentration
250 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to ASH2 immobilized on solid support.

The epitope recognized by IHC-00110 maps to a region between residue 575 and the C-terminus (residue 628) of human Absent, Small, or Homeotic-Like 2 using the numbering given in Swiss-Prot entry Q9UBL3 (GeneID 9070).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to ASH2 immobilized on solid support. The epitope recognized by IHC-00110-T maps to a region between residue 575 and the C-terminus (residue 628) of human Absent, Small, or Homeotic-Like 2 using th
Country of Origin
USA

Additional Product Information

ASH2 (absent, small, or homeotic discs 2) is a subunit of a human Set1-like histone methyltransferase (HMT) complex. The Set-1 like complexes are responsible for mono-, di-, and tri-methylation of histone H3 K4. ASH2 associates with a multitude of proteins in a number of Set1-like complexes which include various combinations of MLL, MLL2, MLL3, MLL4, RBBP5,WDR5, hDPY-30, hSwd2, CXXC1, HCF1, Menin, PTIP, PA1, NCOA6, and UTX.

9070
ASH2L
ASH2 like, histone lysine methyltransferase complex subunit
Q9UBL3
Set1/Ash2 histone methyltransferase complex subunit ASH2

Alternate Names

ASH2; ash2 (absent, small, or homeotic)-like; ASH2L1; ASH2L2; ASH2-like protein; Bre2; set1/Ash2 histone methyltransferase complex subunit ASH2

Applications

Epitope exposure is recommended.

Epitope exposure with citrate buffer will enhance staining.

Likely to work with frozen sections.

In some cases, the antibody may be diluted further than indicated.