Rabbit anti-ASXL2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-037A maps to a region between residue 600 and 650 of human additional sex combs like 2 using the numbering given in entry NP_060733.4 (GeneID 55252).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to ASXL2 immobilized on solid support.
The epitope recognized by A302-037A-T maps to a region between residue 600 and 650 of human additional sex combs like 2 using the numbering given in entry NP_060733.4 (GeneID 55252).
Additional Product Information
The gene that encodes Additional sex combs-like protein 2 (ASXL2) is a homolog of the drosophila additional sex combs (Asx) gene. Drosophila Asx is in a class of proteins known as ETP (enhancers of trithorax and polycomb). ETP proteins regulate polycomb-group (PcG) and trithorax-group (trxG) proteins which function to modify the methylation of histones and structure of chromatin. Mutations in ASXL1 are implicated in the development of myelodysplastic syndromes (MDS), a group of hematological diseases characterized by ineffective hematopoiesis and a predisposition to acute myeloid leukemia (AML).
Alternate Names
additional sex combs like 2, transcriptional regulator; additional sex combs-like protein 2; ASXH2; polycomb group protein ASXH2; putative Polycomb group protein ASXL2; SHAPNS
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.