Rabbit anti-ATF7 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-431A maps to a region between residue 444 and 494 of human activating transcription factor 7 using the numbering given in entry CAA40483.1 (GeneID 11016).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to ATF7 immobilized on solid support.
The epitope recognized by A302-431A-T maps to a region between residue 444 and 494 of human activating transcription factor 7 using the numbering given in entry CAA40483.1 (GeneID 11016).
Additional Product Information
Activating transcription factor 7 (ATF7) is homologous to ATF2. ATF7 was identified as a protein that interacts with the PRL-1 protein-tyrosine phosphatase. It is a basic leucine zipper (bZIP) and C2H2- type zinc-finger containing transcription factor that binds to consensus cAMP response elements (CRE). ATF7 interacts with TAF12, a subunit of the TFIID general transcription factor, to promote transcription.
Alternate Names
Activating transcription factor 7; ATFA; cAMP-dependent transcription factor ATF-7; cyclic AMP-dependent transcription factor ATF-7; transcription factor ATF-A
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.