Rabbit anti-B7-H3/CD276 Recombinant Monoclonal Antibody [BLR026F]
Product Details
Specifications
Immunogen was a recombinant protein representing the extracellular domain of human CD276 (residues 29-465, NP_001019907.1).
Additional Product Information
CD276 may participate in the regulation of T-cell-mediated immune response. CD276 may play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. It may also be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces and could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q5ZPR3].
Alternate Names
4Ig-B7-H3; B7 homolog 3; B7H3; B7-H3; B7RP-2; CD antigen CD276; CD276 antigen; costimulatory molecule
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.