Rabbit anti-BAG3 Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
BAG3 (BCL2-associated athanogene 3) BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. BAG3 contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All three proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner [taken from NCBI Entrez Gene (Gene ID: 9531)].
Alternate Names
BAG family molecular chaperone regulator 3; BAG-3; Bcl-2-associated athanogene 3; BCL2-binding athanogene 3; bcl-2-binding protein Bis; BIS; CAIR-1; docking protein CAIR-1; MFM6
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.