Rabbit anti-BCAR1/p130CAS IHC Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by IHC-00598 maps to a region between residue 275 and 325 of human breast cancer anti-estrogen resistance 1 (Crk-associated substrate p130Cas) using the numbering given in entry NP_055382.2 (GeneID 9564).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to BCAR1/p130CAS immobilized on solid support.
The epitope recognized by IHC-00598-T maps to a region between residue 275 and 325 of human breast cancer anti-estrogen resistance 1 (Crk-associated substrate p130Cas) using the numbering given in entry NP_055382.2 (GeneID 9564).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
BCAR1/p130CAS functions as a scaffolding protein for components of various kinase signaling pathways. It is involved in integrin signaling for cell motility as well as netrin signaling for the guidance of commissural axons. Overexpression of BCAR1/p130CAS has been shown to confer antiestrogen resistance in breast cancer cells.
Alternate Names
BCAR1, Cas family scaffolding protein; breast cancer anti-estrogen resistance protein 1; CAS; Cas scaffolding protein family member 1; CAS1; CASS1; CRKAS; CRK-associated substrate; Crk-associated substrate p130Cas; P130Cas
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.