Rabbit anti-BLOC1S3 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-612A maps to a region between residue 1 to 50 of human Biogenesis of Lysosomal Organelles Complex-1, Subunit 3 using the numbering given in entry NP_997715.1 (GeneID 388552).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to BLOC1S3 immobilized on solid support.
The epitope recognized by A304-612A-T maps to a region between residue 1 to 50 of human Biogenesis of Lysosomal Organelles Complex-1, Subunit 3 using the numbering given in entry NP_997715.1 (GeneID 388552).
Additional Product Information
BLOC1S3 is a component of the BLOC-1 complex, a complex that is required for normal biogenesis of lysosome-related organelles (LRO), such as platelet dense granules and melanosomes. In concert with the AP-3 complex, the BLOC-1 complex is required to target membrane protein cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. The BLOC-1 complex, in association with SNARE proteins, is also proposed to be involved in neurite extension. It plays a role in intracellular vesicle trafficking [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q6QNY0].
Alternate Names
biogenesis of lysosome-related organelles complex 1 subunit 3; biogenesis of lysosome-related organelles complex-1, subunit 3; BLOC-1 subunit 3; BLOS3; HPS8; reduced pigmentation, mouse, homolog of; RP
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.