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Rabbit anti-BLVRB/Biliverdin reductase B Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
BLVRB/Biliverdin reductase B
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 1 and 50
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to BLVRB/Biliverdin reductase B immobilized on solid support.

The epitope recognized by A305-569A maps to a region between residue 25 to 75 of human Flavin reductase using the numbering given in entry P30043.3 (GeneID 645).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to BLVRB/Biliverdin reductase B immobilized on solid support.

The epitope recognized by A305-569A-T maps to a region between residue 25 to 75 of human Flavin reductase using the numbering given in entry P30043.3 (GeneID 645).

Country of Origin
USA

Additional Product Information

The final step in heme metabolism in mammals is catalyzed by the cytosolic biliverdin reductase enzymes A and B (EC 1.3.1.24) [taken from NCBI Entrez Gene (Gene ID: 645)].

645
BLVRB
biliverdin reductase B
P30043
Flavin reductase

Alternate Names

Biliverdin reductase B; biliverdin-IX beta-reductase; BVRB; BVR-B; epididymis secretory protein Li 10; flavin reductase (NADPH); FLR; FR; GHBP; green heme-binding protein; HEL-S-10; NADPH; NADPH-dependent diaphorase; NADPH-flavin reductase; SDR43U1; short chain dehydrogenase/reductase family 43U, member 1

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.