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Rabbit anti-BOLA2 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
BOLA2
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 36 and C-terminus
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to BOLA2 immobilized on solid support.

The epitope recognized by A305-890A maps to a region between residue 36 and 86 of human BolA-like protein 2 using the numbering given in entry Q9H3K6.1 (GeneID 552900).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to BOLA2 immobilized on solid support.

The epitope recognized by A305-890A-T maps to a region between residue 36 and 86 of human BolA-like protein 2 using the numbering given in entry Q9H3K6.1 (GeneID 552900).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA

Additional Product Information

BOLA2 acts as a cytosolic iron-sulfur (Fe-S) cluster assembly factor that facilitates [2Fe-2S] cluster insertion into a subset of cytosolic proteins. Acts together with the monothiol glutaredoxin GLRX3. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9H3K6].

552900
BOLA2
bolA family member 2
Q9H3K6
BolA-like protein 2

Alternate Names

bolA homolog 2; BOLA2A; BOLA2B; bolA-like protein 2; BolA-like protein 2 member A; My016

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.