Rabbit anti-BORG4 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-380A-T maps to a region between residue 250 and 300 of human binder of Rho GTPases 4 using the numbering given in entry NP_036253.2 (GeneID 23580).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to BORG4 immobilized on solid support.
The epitope recognized by A302-380A maps to a region between residue 250 and 300 of human binder of Rho GTPases 4 using the numbering given in entry NP_036253.2 (GeneID 23580).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
BORG4 (binder of Rho GTPases 4) is part of a family of proteins that function as negative regulators of CDC42 Rho GTPase signaling. The Borg family of proteins are also known as CEPs (Cdc effector proteins) and have been shown to act downstream of Cdc42 to influence the assembly of actin filaments and changes in cell shape.
Alternate Names
binder of Rho GTPases 4; BORG4; CDC42 effector protein (Rho GTPase binding) 4; cdc42 effector protein 4; CEP4; KAIA1777
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.