Rabbit anti-BRCC36 IHC Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by IHC-00715 maps to a region between residue 266 and 316 of human BRCA1/BRCA2-containing complex subunit 36 using the numbering given in entry NP_077308.1 (GeneID 79184).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to BRCC36 immobilized on solid support.
The epitope recognized by IHC-00715-T maps to a region between residue 266 and 316 of human BRCA1/BRCA2-containing complex subunit 36 using the numbering given in entry NP_077308.1 (GeneID 79184).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
BRCA1/BRCA2-containing complex subunit 36 (BRCC36) is a metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. BRCC36 is a component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites and leads to targeting of the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). In the BRCA1-A complex, BRCC36 specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double-strand breaks (DSBs) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P46736].
Alternate Names
BRCA1/BRCA2-containing complex subunit 3; BRCA1/BRCA2-containing complex subunit 36; BRCA1-A complex subunit BRCC36; BRCC36; BRISC complex subunit BRCC36; C6.1A; CXorf53; lys-63-specific deubiquitinase BRCC36
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.