Rabbit anti-BRF1 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
BRF1
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 627 and C-terminus
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (200 µg/ml)

10 µl (200 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Mouse, Human
Antigen Species
Human
Concentration
200 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to BRF1 immobilized on solid support.

The epitope recognized by A301-228A maps to a region between residue 627 and 677 of human B - related factor 1 using the numbering given in entry NP_001510.2 (GeneID 2972).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to BRF1 immobilized on solid support.

The epitope recognized by A301-228A-T maps to a region between residue 627 and 677 of human B - related factor 1 using the numbering given in entry NP_001510.2 (GeneID 2972).
Country of Origin
USA

Additional Product Information

BRF1 is one of three subunits of the RNA polymerase III transcription factor complex, TFIIIB. TFIIIB regulates RNA Polymerase III transcription which manufactures tRNAs, 5S ribosomal RNA and several other untranslated RNAs.

2972
BRF1
BRF1, RNA polymerase III transcription initiation factor subunit
Q92994
Transcription factor IIIB 90 kDa subunit

Alternate Names

B-related factor 1; BRF; BRF-1; BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB; BRF1, RNA polymerase III transcription initiation factor 90 kDa subunit; CFDS; epididymis secretory sperm binding protein Li 76p; general transcription factor IIIB, 90kD subunit; GTF3B; hBRF; HEL-S-76p; hTFIIIB90; TAF3B2; TAF3C; TAFIII90; TATA box binding protein (TBP)-associated factor 3C; TATA box-binding protein-associated factor, RNA polymerase III, subunit 2; TBP - associated factor, RNA polymerase III, 90kD; TF3B90; TFIIIB90; transcription factor IIIB 90 kDa subunit

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.

Immunohistochemistry (IHC)
1:200 - 1:1000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)
6 µg/mg lysate
Western Blot (WB)
1:2000 - 1:10,000