Rabbit anti-BRG1 Recombinant Monoclonal Antibody [BLR106H]
Product Details
Specifications
Request Formulation Change Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
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Immunogen was a peptide representing a region between residue 75 and 125 of human transcription activator BRG1 using the numbering given in entry NP_001122321.1 (Gene ID 6597).
Additional Product Information
The SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin (SMARC), also called BRG1-associated factors (BAFs), have been identified as components of the human SWI/SNF-like chromatin-remodeling protein complexes. BRG1/SMARCA4 is an ATPase subunit of the SWI/SNF-A chromatin remodeling complex (BAF complex). In BAF complexes, BRG1/SMARCA4 has been shown to interact with BRCA1.
Alternate Names
ATP-dependent helicase SMARCA4; BAF190; BAF190A; brahma protein-like 1; BRG1; BRG1-associated factor 190A; BRM/SWI2-related gene 1; CSS4; global transcription activator homologous sequence; homeotic gene regulator; hSNF2b; mitotic growth and transcription activator; MRD16; nuclear protein GRB1; protein brahma homolog 1; protein BRG-1; RTPS2; SNF2; SNF2-beta; SNF2L4; SNF2LB; SNF2-like 4; sucrose nonfermenting-like 4; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4; SWI2; transcription activator BRG1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.