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Rabbit anti-c-Jun Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
c-Jun
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 25 and 75
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Mouse, Human
Presumed Reactivity
Bovine
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to c-Jun immobilized on solid support.

The epitope recognized by A302-959A maps to a region between residue 40 and 80 of human Jun Oncogene using the numbering given in entry NP_002219.1 (GeneID 3725).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to c-Jun immobilized on solid support.

The epitope recognized by A302-959A-T maps to a region between residue 40 and 80 of human Jun Oncogene using the numbering given in entry NP_002219.1 (GeneID 3725).
Country of Origin
USA

Additional Product Information

c-Jun is a transcription factor that heterodimerizes with FOS to form the activator protein 1 (AP-1) transcription factor. The JUN gene is the putative transforming gene of avian sarcoma virus 17.

3725
JUN
Jun proto-oncogene, AP-1 transcription factor subunit
P05412
Transcription factor AP-1

Alternate Names

activator protein 1; AP1; AP-1; c-Jun; enhancer-binding protein AP1; Jun activation domain binding protein; jun oncogene; p39; proto-oncogene c-Jun; transcription factor AP-1; v-jun avian sarcoma virus 17 oncogene homolog; v-jun sarcoma virus 17 oncogene homolog

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.