Rabbit anti-c-Myb Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-135A-T maps to a region between residue 1 to 50 of human V-myb Myeloblastosis Viral Oncogene Homolog using the numbering given in entry NP_005366.2 (GeneID 4602).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to c-Myb immobilized on solid support.
The epitope recognized by A304-135A maps to a region between residue 1 to 50 of human V-myb Myeloblastosis Viral Oncogene Homolog using the numbering given in entry NP_005366.2 (GeneID 4602).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
c-Myb is a member of the MYB family of transcription factors. C-Myb contains three domains, an N-terminal DNA-binding domain, a central transcriptional activation domain and a C-terminal domain involved in transcriptional repression. C-myb plays an essential role in the regulation of hematopoiesis and may play a role in tumorigenesis [taken from NCBI Entrez Gene (Gene ID: 3093)].
Alternate Names
Cmyb; c-myb; c-myb_CDS; efg; oncogene AMV; proto-oncogene c-Myb; transcriptional activator Myb; v-myb avian myeloblastosis viral oncogene homolog
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.