Rabbit anti-Calpastatin/CAST Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-794A maps to a region between residue 658 to 708 of human Calpastatin using the numbering given in entry P20810 (GeneID 831).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Calpastatin/CAST immobilized on solid support.
The epitope recognized by A304-794A-T maps to a region between residue 658 to 708 of human Calpastatin using the numbering given in entry P20810 (GeneID 831).
Additional Product Information
Calpastatin is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation [taken from NCBI Entrez Gene (Gene ID: 831)].
Alternate Names
BS-17; calpain inhibitor; calpastatin; PLACK; sperm BS-17 component
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.