Rabbit anti-CaMKK alpha Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-669A maps to a region between residue 455 and 505 of human calcium/calmodulin-dependent protein kinase kinase 1, alpha using the numbering given in entry NP_757343.1 (GeneID 84254).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to CaMKK alpha immobilized on solid support.
The epitope recognized by A302-669A-T maps to a region between residue 455 and 505 of human calcium/calmodulin-dependent protein kinase kinase 1, alpha using the numbering given in entry NP_757343.1 (GeneID 84254).
Additional Product Information
CaMKK alpha is a calcium/calmodulin-dependent protein kinase that phosphorylates CAMK1, CAMK1D, CAMK1G and CAMK4. CaMKK alpha is involved in regulating cell apoptosis. It promotes cell survival by phosphorylating AKT1/PKB that inhibits pro-apoptotic BAD/Bcl2-antagonist of cell death [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q8N5S9].
Alternate Names
calcium/calmodulin-dependent protein kinase kinase 1; calcium/calmodulin-dependent protein kinase kinase 1, alpha; calcium/calmodulin-dependent protein kinase kinase alpha; caM-kinase IV kinase; caM-kinase kinase 1; caM-kinase kinase alpha; caMKK 1; caM-KK 1; CaMKK alpha; caM-KK alpha; CAMKK alpha protein; CAMKKA
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.