Rabbit anti-CAR Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-848A maps to a region between residue 335 and 365 of human Coxsackievirus and Adenovirus Receptor Protein using the numbering given in entry NP_001329.1 (GeneID 1525).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to CAR immobilized on solid support.
The epitope recognized by A302-848A-T maps to a region between residue 335 and 365 of human Coxsackievirus and Adenovirus Receptor Protein using the numbering given in entry NP_001329.1 (GeneID 1525).
Additional Product Information
Coxsackievirus and adenovirus receptor (CAR) is a component of the epithelial apical junction complex that is essential for the integrity of tight junctions. CAR is proposed to function as a homophilic cell adhesion molecule. It is also thought to be involved in transepithelial migration of polymorphonuclear leukocytes (PMN) through adhesive interactions with AMICA1/JAML located in the plasma membrane of PMN [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P78310].
Alternate Names
46 kD coxsackievirus and adenovirus receptor (CAR) protein; CAR; CAR4/6; coxsackie virus and adenovirus receptor; coxsackievirus and adenovirus receptor; coxsackievirus B-adenovirus receptor; CVB3-binding protein; HCAR; HCVADR
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.