Rabbit anti-CARMIL2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-831A maps to a region between residue 1385 and 1435 of human Leucine-rich repeat-containing protein 16C using the numbering given in entry Q6F5E8.2 (GeneID 146206).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to CARMIL2 immobilized on solid support.
The epitope recognized by A305-831A-T maps to a region between residue 1385 and 1435 of human Leucine-rich repeat-containing protein 16C using the numbering given in entry Q6F5E8.2 (GeneID 146206).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Cell membrane-cytoskeleton-associated protein that plays a role in the regulation of actin polymerization at the barbed end of actin filaments. Prevents F-actin heterodimeric capping protein (CP) activity at the leading edges of migrating cells, and hence generates uncapped barbed ends and enhances actin polymerization. Plays a role in cell protrusion formations; involved in cell polarity, lamellipodial assembly, membrane ruffling and macropinosome formations. Involved as well in cell migration and invadopodia formation during wound healing. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q6F5E8].
Alternate Names
Capping protein regulator and myosin 1 linker 2; capping protein, Arp2/3 and myosin-I linker protein 2; CARMIL2b; F-actin-uncapping protein RLTPR; IMD58; leucine rich repeat containing 16C; leucine-rich repeat-containing protein 16C; LRRC16C; RGD motif, leucine rich repeats, tropomodulin domain and proline-rich containing; RGD, leucine-rich repeat, tropomodulin and proline-rich containing protein; RGD, leucine-rich repeat, tropomodulin and proline-rich-containing protein; RLTPR
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.