Rabbit anti-Cbl-b Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-903A maps to a region between residue 932 and 982 of human Cas-Br-M (murine) Ecotropic Retroviral Transforming Sequence b using the numbering given in entry NP_733762.2 (GeneID 868).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Cbl-b immobilized on solid support.
The epitope recognized by A302-903A-T maps to a region between residue 932 and 982 of human Cas-Br-M (murine) Ecotropic Retroviral Transforming Sequence b using the numbering given in entry NP_733762.2 (GeneID 868).
Additional Product Information
Cbl-b is an E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Cbl-b negatively regulates TCR (T-cell receptor), BCR (B-cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q13191].
Alternate Names
Cas-Br-M (murine) ecotropic retroviral transforming sequence b; casitas B-lineage lymphoma proto-oncogene b; Cbl proto-oncogene B, E3 ubiquitin protein ligase; Cbl proto-oncogene, E3 ubiquitin protein ligase B; Cbl-b; E3 ubiquitin-protein ligase CBL-B; Nbla00127; RING finger protein 56; RING-type E3 ubiquitin transferase CBL-B; RNF56; SH3-binding protein CBL-B; signal transduction protein CBL-B
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.