Rabbit anti-CBP Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-363A-T maps to a region between residues 2375 and 2425 of human CREB Binding Protein using the numbering given in entry NP_004371.1 (GeneID 1387). Antibody was affinity purified using an epitope specific to CBP immobilized on solid support.
The epitope recognized by A300-363A maps to a region between residues 2375 and 2425 of human CREB Binding Protein using the numbering given in entry NP_004371.1 (GeneID 1387).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
CBP [cAMP response element-binding protein (CREB)-binding protein] interacts with and is a transcriptional co-activator of a multitude of transcriptional regulators that includes CREB. CBP is structurally and functionally similar to p300 but is encoded by an independent gene. CBP coactivation may be achieved through the regulation of chromatin structure due to its ability to catalyze the acetylation of histones. CBP has potential tumor suppressor properties and is important to integrating cell-signaling cues for proliferation, differentiation and apoptosis.
Alternate Names
CBP; CREB-binding protein; KAT3A; RSTS; RSTS1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.