Rabbit anti-CCAR1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-435A maps to a region between residues 1 and 50 of human Cell-Cycle and Apoptosis Regulatory Protein 1 using the numbering given in TrEMBL entry Q6X935 (GeneID 55749).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to CCAR1 immobilized on solid support.
The epitope recognized by A300-435A-T maps to a region between residues 1 and 50 of human Cell-Cycle and Apoptosis Regulatory Protein 1 using the numbering given in TrEMBL entry Q6X935 (GeneID 55749).
Additional Product Information
CCAR1 was identified as a perinuclear phosphoprotein that regulates apoptosis signals in response to chemotherapeutic agents. Induction of apoptosis by CCAR1 involves the epidermal growth factor receptor (EGFR), mitogen-activated protein kinase pathway and caspase-9 activation. CARP-1 has also been demonstrated as a suppressor of human breast cancer growth.
Alternate Names
CARP-1; cell cycle and apoptosis regulatory protein 1; cell division cycle and apoptosis regulator protein 1; death inducer with SAP domain
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.