Rabbit anti-CCDC94 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-882A maps to a region between residue 273 and 323 of human Coiled-coil domain-containing protein 94 using the numbering given in entry Q9BW85.1 (GeneID 55702).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to CCDC94 immobilized on solid support.
The epitope recognized by A305-882A-T maps to a region between residue 273 and 323 of human Coiled-coil domain-containing protein 94 using the numbering given in entry Q9BW85.1 (GeneID 55702).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
CCDC94 is part of the spliceosome which catalyzes two sequential transesterification reactions, first the excision of the non-coding intron from pre-mRNA and then the ligation of the coding exons to form the mature mRNA. CCDC94 also plays a role in stabilizing the structure of the spliceosome catalytic core and docking of the branch helix into the active site, producing 5'-exon and lariat intron-3'-intermediates. CCDC94 may protect cells from TP53-dependent apoptosis upon dsDNA break damage through association with PRP19-CD5L complex. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9BW85].
Alternate Names
CCDC94; coiled-coil domain containing 94; coiled-coil domain-containing protein 94; YJU2 splicing factor homolog
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.