Rabbit anti-CCT8 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-447A maps to a region between residue 398 and 448 of human Chaperonin Containing TCP1, Subunit 8 using the numbering given in entry NP_006576.2 (GeneID 10694).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to CCT8 immobilized on solid support.
The epitope recognized by A303-447A-T maps to a region between residue 398 and 448 of human Chaperonin Containing TCP1, Subunit 8 using the numbering given in entry NP_006576.2 (GeneID 10694).
Additional Product Information
CCT8 is also known as T-complex protein 1 subunit theta (TCP1-theta) and is a molecular chaperone that assists in the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex, CCT8 may play a role in the assembly of BBSome, a complex involved in ciliogenesis and regulating the transport of vesicles to the cilia. TCP1-theta is known to play a role, in vitro, in the folding of actin and tubulin [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ P50990].
Alternate Names
C21orf112; Cctq; CCT-theta; chaperonin containing T-complex polypeptide 1 subunit 8; chaperonin containing TCP1, subunit 8 (theta); D21S246; PRED71; renal carcinoma antigen NY-REN-15; T-complex protein 1 subunit theta; TCP-1-theta
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.