/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-CD22 Recombinant Monoclonal Antibody [BLR320M]
Primary Antibodies  

Rabbit anti-CD22 Recombinant Monoclonal Antibody [BLR320M]

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

CD22

Reactivity:

Mouse

Applications:

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Recombinant Monoclonal

Clone:

BLR320M

Format:

Whole IgG

Immunogen:

between 750 and 800

Isotype:

IgG

Purity:

Purified

100 µl (50+ tests)

10 µl (5+ tests)

$127.00 $482.00
Qty:
Bulk Request

Product Details

Specifications
Verified Reactivity

Mouse

Antigen Species

Mouse

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Phosphate Buffered Saline (PBS) with 0.09% Sodium Azide, BSA-Free


Request Formulation Change
Production Procedures

Recombinant antibody was purified from cell culture supernatant.

Immunogen was a peptide representing a region between residue 750 and 800 of mouse B-cell receptor CD22 using the numbering given in entry NP_001036782.1 (Gene ID 12483).

Additional Product Information

CD22 mediates B-cell B-cell interactions and is involved in the localization of B-cells in lymphoid tissues. CD22 binds sialylated glycoproteins including CD45. It is involved in B cell activation; negative regulation of the B cell receptor signaling pathway; and regulation of endocytosis. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P20273].

12483

Cd22

CD22 antigen

P35329

B-cell receptor CD22

Alternate Names

A530093D23;B-cell receptor CD22;BL-CAM;B-lymphocyte cell adhesion molecule;CD antigen CD22;Lyb8;Lyb-8;sialic acid-binding Ig-like lectin 2;siglec-2;T-cell surface antigen Leu-14

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.


Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).


Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (A120-113P) with 5% Normal Pig Serum (S100-020) added to the blocking buffer.