Rabbit anti-CD247/CD3Z Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-171A maps to a region between residue 114 to 164 of human T-cell surface glycoprotein CD3 zeta chain using the numbering given in entry P20963.2 (GeneID 919).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to CD247/CD3Z immobilized on solid support.
The epitope recognized by A305-171A-T maps to a region between residue 114 to 164 of human T-cell surface glycoprotein CD3 zeta chain using the numbering given in entry P20963.2 (GeneID 919).
Additional Product Information
T-cell surface glycoprotein CD3 zeta chain is encoded by the gene CD247, also known as CD3Z or CD3 zeta. CD247/CD3 zeta, together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response [taken from NCBI Entrez Gene (Gene ID: 919)].
Alternate Names
CD antigen CD247; CD247 antigen, zeta subunit; CD3H; CD3Q; CD3; ZCD3Z antigen, zeta polypeptide (TiT3 complex); CD3-ZETA; CD3zeta chain; IMD25; T3; ZT-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor T3 zeta chain; T-cell surface glycoprotein CD3 zeta chain; TCR zeta chain; TCRZ
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.