Rabbit anti-CD4 Recombinant Monoclonal Antibody [BLR167J]
Product Details
Specifications
Immunogen was a peptide representing a region between residue 407 and the C-terminus (residue 457) of mouse T-cell surface glycoprotein CD4 using the numbering given in entry NP_038516.1 (Gene ID 12504).
Additional Product Information
CD4 is a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. CD4 is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system [taken from NCBI Entrez Gene (Gene ID: 920)].
Alternate Names
CD antigen CD4; L3T4; Ly-4; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.