Rabbit anti-CD86 Recombinant Monoclonal Antibody [BLR255L]
Product Details
Specifications
Immunogen was a recombinant protein representing the extracellular domain of mouse T-lymphocyte activation antigen CD86 (residues 24-245, NP_062261.3).
Additional Product Information
CD86 is a type I membrane protein that is a member of the immunoglobulin superfamily. CD86 is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response [taken from NCBI Entrez Gene (Gene ID: 942)].
Alternate Names
activation B7-2 antigen; B7; B7.2; B70; B7-2; CD antigen CD86; Cd28l2; CLS1; early T cell costimulatory molecule-1; Early T-cell costimulatory molecule 1; ETC-1; Ly58; Ly-58; MB7; MB7-2; T-lymphocyte activation antigen CD86; TS/A-2
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.