Rabbit anti-CDC37 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-489A maps to a region between residue 324 to 374 of human cell division cycle 37 using the numbering given in entry NP_008996.1 (GeneID 11140).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to CDC37 immobilized on solid support.
The epitope recognized by A302-489A-T maps to a region between residue 324 to 374 of human cell division cycle 37 using the numbering given in entry NP_008996.1 (GeneID 11140).
Additional Product Information
Cell division cycle 37 protein (CDC37) is also known as Hsp90 co-chaperone Cdc37. CDC37 is highly similar to Cdc37, a cell division cycle control protein of Saccharomyces cerevisiae. This protein is a molecular chaperone with specific function in cell signal transduction. It has been shown to form a complex with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF-1, MOK, as well as eIF2 alpha kinases. It is thought to play a critical role in directing Hsp90 to its target kinases [taken from NCBI Entrez Gene (GeneID: 11140)].
Alternate Names
CDC37 (cell division cycle 37, S. cerevisiae, homolog); CDC37 cell division cycle 37 homolog; cell division cycle 37 homolog; hsp90 chaperone protein kinase-targeting subunit; hsp90 co-chaperone Cdc37; P50CDC37
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.