Rabbit anti-CDK4 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-224A maps to a region between residue 1 to 50 of human Cyclin-Dependent Kinase 4 using the numbering given in entry NP_000066.1 (GeneID 1019).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to CDK4 immobilized on solid support.
The epitope recognized by A304-224A-T maps to a region between residue 1 to 50 of human Cyclin-Dependent Kinase 4 using the numbering given in entry NP_000066.1 (GeneID 1019).
Additional Product Information
Cyclin dependent kinase 4 (CDK4) is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of CDK4 is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in the CDK4 gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers [taken from NCBI Entrez Gene (Gene ID: 1019)].
Alternate Names
cell division protein kinase 4; CMM3; cyclin-dependent kinase 4; PSK-J3
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).