Rabbit anti-CENP-F/Mitosin Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-612A-T maps to a region between residue 3150 and 3200 of human centromere protein F (Mitosin) using the numbering given in entry AAA82889.1 (GeneID 1063).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to CENP-F/Mitosin immobilized on solid support.
The epitope recognized by A301-612A maps to a region between residue 3150 and 3200 of human centromere protein F (Mitosin) using the numbering given in entry AAA82889.1 (GeneID 1063).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Centromere protein F (CENP-F) is a microtubule binding protein and component of the kinetochore. It is essential for kinetochore attachment, chromosome alignment, and the spindle checkpoint. CENP-F appears to be important to the association of the NudE-related proteins Ndel1 and Nde1 to the Lis1/dynein motor complex and their role in kinetochore function. CENP-F is a farnesylated protein and the farnesylation of CENP-F appears to be central to its role in mediating kinetochore-microtubule interactions as well as its degradation after mitosis. Recently, CENP-F expression has been correlated with markers of poor outcome in breast cancer.
Alternate Names
AH antigen; cell-cycle-dependent 350K nuclear protein; CENF; CENP-F; CENP-F kinetochore protein; centromere protein F; centromere protein F, 350/400kDa; CILD31; hcp-1; kinetochore protein CENPF; mitosin; PRO1779; STROMS
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.