Rabbit anti-CENP-F/Mitosin IHC Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by IHC-00065 maps to a region between residues 1800 and 1850 of human Centromere Protein F using the numbering given in SwissProt entry P49454 (GeneID 1063). Antibody was affinity purified using an epitope specific to CENP-F/Mitosin immobilized on solid support.
The epitope recognized by IHC-00065-T maps to a region between residues 1800 and 1850 of human Centromere Protein F using the numbering given in SwissProt entry P49454 (GeneID 1063).
Additional Product Information
Centromere protein F (CENP-F) is a microtubule binding protein and component of the kinetochore. It is essential for kinetochore attachment, chromosome alignment, and the spindle checkpoint. CENP-F appears to be important to the association of the NudE-related proteins Ndel1 and Nde1 to the Lis1/dynein motor complex and their role in kinetochore function. CENP-F is a farnesylated protein and the farnesylation of CENP-F appears to be central to its role in mediating kinetochore-microtubule interactions as well as its degradation after mitosis. Recently, CENP-F expression has been correlated with markers of poor outcome in breast cancer.
Alternate Names
AH antigen; cell-cycle-dependent 350K nuclear protein; CENF; CENP-F; CENP-F kinetochore protein; centromere protein F; centromere protein F, 350/400kDa; CILD31; hcp-1; kinetochore protein CENPF; mitosin; PRO1779; STROMS
Applications
Acetone-Fixed Cells 1:100 - 1:500:
Formaldehyde or methanol fixation is not recommended.