Rabbit anti-CEP76 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
CEP76
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 1 and 50
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Presumed Reactivity
Mouse
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to CEP76 immobilized on solid support.

The epitope recognized by A302-325A maps to a region between residue 1 and 30 of human centrosomal protein 76kDa using the numbering given in entry NP_079175.2 (GeneID 79959).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to CEP76 immobilized on solid support.

The epitope recognized by A302-325A-T maps to a region between residue 1 and 30 of human centrosomal protein 76kDa using the numbering given in entry NP_079175.2 (GeneID 79959).
Country of Origin
USA

Additional Product Information

Centrosomoal protein of 76 kDa (CEP76) is a centrosomal protein involved in regulation of centriole duplication. It is required to limit centriole duplication to once per cell cycle by preventing centriole reduplication [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q8TAP6].

79959
CEP76
centrosomal protein 76
Q8TAP6
Centrosomal protein of 76 kDa

Alternate Names

C18orf9; centrosomal protein 76kDa; centrosomal protein of 76 kDa; Cep76; HsT1705

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.

Immunoprecipitation (IP)
6 µg/mg lysate
Western Blot (WB)
1:2000 - 1:10,000