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Rabbit anti-CEP97 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
CEP97
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 675 and 725
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to CEP97 immobilized on solid support.

The epitope recognized by A301-946A maps to a region between residue 675 and 725 of human centrosomal protein 97kDa using the numbering given in entry NP_078824.2 (GeneID 79598).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to CEP97 immobilized on solid support.

The epitope recognized by A301-946A-T maps to a region between residue 675 and 725 of human centrosomal protein 97kDa using the numbering given in entry NP_078824.2 (GeneID 79598).
Country of Origin
USA

Additional Product Information

CEP97 (centrosomal protein of 97 kDa) has been identified as part of a complex with CP110. Together, CP110 and CEP97 have been shown to play a role in the assembly of cilia. Loss of CEP97 or CEP110 promotes cilia formation while forced expression inhibits ciliogenesis.

79598
CEP97
centrosomal protein 97
Q8IW35
Centrosomal protein of 97 kDa

Alternate Names

2810403B08Rik; centrosomal protein of 97 kDa; Cep97; leucine-rich repeat and IQ domain-containing protein 2; leucine-rich repeats and IQ motif containing 2; LRRIQ2

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.