Rabbit anti-ch-TOG Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-971A maps to a region between residue 1982 and 2032 of human colonic and hepatic tumor over-expressed protein using the numbering given in entry NP_001008938.1 (GeneID 9793).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to ch-TOG immobilized on solid support.
The epitope recognized by A301-971A-T maps to a region between residue 1982 and 2032 of human colonic and hepatic tumor over-expressed protein using the numbering given in entry NP_001008938.1 (GeneID 9793).
Additional Product Information
Colonic and hepatic tumor over-expressed protein (ch-TOG) is a protein originally described as a gene over-expressed in human hepatomas and colonic tumors. It was found to be a homolog of the xenopus egg protein, XMAP214, a microtubule associated protein (MAP). The human ch-TOG protein has been shown to promote microtubule assembly and is a major component of microtubule asters. It is required for centrosome integrity, spindle pole organization and bipolar spindle assembly.
Alternate Names
CHTOG; ch-TOG; colonic and hepatic tumor overexpressed gene protein; colonic and hepatic tumor over-expressed gene protein; cytoskeleton-associated protein 5; MSPS; TOG; TOGp
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.