Rabbit anti-CHD8 IHC Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by IHC-00246 maps to a region between residue 2252 and 2302 of human chromodomain helicase DNA binding protein 8 using the numbering given in entry NP_065971.2 (GeneID 57680).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to CHD8 immobilized on solid support.
The epitope recognized by IHC-00246-T maps to a region between residue 2252 and 2302 of human chromodomain helicase DNA binding protein 8 using the numbering given in entry NP_065971.2 (GeneID 57680).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
CHD8 is a member of the CHD (chromodomain-helicase-DNA-binding) family of proteins that interacts with nucleosomes and plays a role in chromatin remodeling to modulate transcription. The members of the CHD family of proteins possess 3 common structural and functional domains: a chromodomain (chromatin organization modifier), an SNF2-like helicase/ATPase domain, and a C-terminal DNA-binding domain. CHD8 has been reported to interact with duplin, a protein involved in the wnt signaling pathway, and CTCF, a protein involved in heterochromatin formation.
Alternate Names
ATP-dependent helicase CHD8; AUTS18; axis duplication inhibitor; CHD-8; chromodomain-helicase-DNA-binding protein 8; duplin; helicase with SNF2 domain 1; HELSNF1
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.